Ester Experiment The Acidic Environment > 5. Esterification > Identify data, plan, select equipment and perform a first-hand investigation to prepare an ester using reflux The ester octyl acetate can be prepared using the following procedure: Transfer about 12 mL of octanol and about 10 mL of acetic acid into a flask. Add several boiling chips and about 1 mL of concentrated sulfuric acid to the flask. Attach a condenser tube to the flask and run water through the outer case of the tube. Heat the flask with a Bunsen burner for 30 minutes, such that the mixture is evaporating and condensing but not escaping. Allow the mixture to cool, then transfer it to a separating funnel. Add water to the separating funnel, swirl and allow to stand. The top, non-aqueous layer that forms in the funnel is octyl acetate. Open the tap of the separating funnel and allow the aqueous layer to run out, leaving the octyl acetate remaining.